Services
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Instrumentation and services are available to all researchers inside and outside of the university.
Researchers from the Northwestern University and the University of Chicago are provided internal rates as all UIC users. All other users can access services but at an external academic or external non-academic rate, which is determined using subsidy and market rates.
All users must register their funds before requesting service or scheduling equipment usage.
All users are responsible for their own laboratory safety training to be current during each use of the core facilities.
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Mass Spectrometry Core Services Heading link
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- Exact Mass Analysis for small molecules and peptides
- Protein molecular weight measurement
- Protein/peptide fractionation
- Protein identification
- Global quantitative proteomics by label-free or labeling methods (TMT, iTRAQ, SILAC)
- Post-translational modification (PTM) analysis – phosphorylation, acetylation, oxidation, histone acetylation
- Metal analysis by ICP MS
- Untargeted metabolomics/lipidomics
- Targeted protein/peptide quantification by MRM
- Targeted metabolomics/lipidomics by MRM
- Instrument Training on Qtrap, Orbitrap, MALDI-TOF, Q-TOF and ICP MS
- Method Development on request
Featured Targeted Assay Heading link
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- Bile acids
- Ceramide- Molecular species
- Ceramide-1-Phosphate (C1P)
- Sphingosine – Molecular species (S1P)
- Eicosanoids (prostaglandins, leukotrienes, etc)
- Endocannabinoids (eCBs)
- Free fatty acids
- Short chain fatty acids (SCFA)
- Lysophosphatidic acid (LPA) – Molecular species
- Phosphatidic acid (PA) – Molecular species
- Cholesterol and Oxysterols
- Glycolipids – GM1, GM2, GM3, GT1, GD1
- Phosphatidylinositols (PI, PIP, PIP2, PIP3)
- Sex hormones – Testosterone (T, DHT), estrogen (E1, E2, E3), cortisol, cortisone
- Amino Acids
- Nucleotide and Deoxynucleotide
- Perfluorooctanoic acids
- Catecholamines
- Acyl-CoA
- Polyfluoroalkyl substances (PFAS)
- cGAMP
- Antibiotics
- Herbicides/pesticides
- Primary aromatic amines (PAA)
- Flux
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Service Pricing
| Service | Description | Pricing (Internal) |
|---|---|---|
| S01-Bile Acid Analysis | Quantitative analysis of bile acids in biological samples TCDCA TDCA GCDCA UDCA CA TLCA GLCA CDCA DCA GCA This cost covers a section of 35min Qtrap LC-MS run for data acquisition, a section of 10min labor time and a section of reagent kit 2. Additional fee will be applied if extra sample preparation is needed. | $60.00 |
| S02-Creatinine Measurement in urine | Creatinine measurement for urine samples. | $10.00 |
| S03-ECM proteomics | A part of ECM proteomics pipeline including MSC and RIC cores. For MSC core service, the cost covers a section of 2 hours gradient, a section of Mascot database search and a section of Scaffold analysis. Additional fee will be applied from RIC core. Additional fee will be applied if extra sample preparation is needed. | $160.00 |
| S04-Exact Mass by MALDI-TOF | MALDI TOF MS analysis. Supplied matrices including SA, DI, CHCA, DHB and HPA. Other uncommon matrices will be provided by users with special needs. The cost covers a section of 5min instrument time, 5min labor time and a section of reagent kit2. | $20.00 |
| S05-Exact Mass or MS/MS Analysis by LC/MS on Q-TOF | Perform MS or MS/MS analysis by LC/MS. MS and MS/MS analysis can be conducted for one sample with additional 50% cost. The cost covers a section of a 15min LC/MS gradient on Qtof 6545, a section of 10min of labor time and a section of reagent kit 2. | $30.00 |
| S06-Herbicide Analysis | Assay 1 Glyphosate aminomethyl-phosphonic acid (AMPA) glufosinate (GLU) 3-methylphosphinico-propionic acid (MMPA) Assay 2 Atrazine desethylatrazine (DEA) desisopropylatrazine (DIA) diaminochlorotriazine (DACT) The cost covers a section of 10min Qtrap LC/MS run, a section of 5min labor time and a section of reagent kit2. Each assay will be counted as one service. Additional fee will be applied if extra sample preparation is needed. | $30.00 |
| S07-Histone Analysis | Common Histone (H2, H3, H4, etc.) protein modification quantitative analysis The cost covers a section of sample digestion, a section of desalting, a section of derivatization, a section of 2-hour HF MS data acquisition, a section of Mascot database search and a section of one-hour labor. | $400.00 |
| S08-Instrument Training on ICP | Instrument training on ICP MS. Up to 2 trainees are allowed for each section. The second trainees from the same research group needs to pay additional $450 for the 8-hr instrument time in order to gain access. | $800.00 |
| S09-Instrument Training on MALDI-TOF | Instrument training on MALDI-TOF | $640.00 |
| S10-Instrument Training on Qtof | Instrument training on Agilent Qtof 6545. 8hrs of assisted instrument time is included. Up to 2 trainees are allowed in each section. The 2nd trainees from the same research group needs to pay additional $500 for the 8-hr instrument time in order to gain access. | $900.00 |
| S11-Instrument Training on Qtrap | Instrument training on Qtrap. Up to 2 trainees are allowed for each section. The second trainees from the same research group needs to pay additional $450 for the 8-hr instrument time in order to gain access. | $800.00 |
| S12-Instrument Training on Velos Pro | Instrument training on Orbitrap Velos Pro. 8hrs of assisted instrument time is included. Up to 2 trainees are allowed in each section. The 2nd trainees from the same research group needs to pay additional $600 for the 8-hr instrument time in order to gain access. | $1,000.00 |
| S13-MRM quantitation method | Method development of MRM;based quantitation, including LC and MS optimization of the targeted small molecule and generation of a calibration curve The cost covers a section of 3-hour LC/MS run, a section of 2 hour labor. | $300.00 |
| S14-MRM quantitation method protein | Method development of; based quantitation, including protein digestion, LC and MS optimization of the targeted protein and generation of a calibration curve. The cost covers a section of 3-hour LC/MS run, a section of 2 hour labor, a section of desalting, a section of protein digestion and 4 sections of reagent kit 2. | $450.00 |
| S15-MRM Standard Curve | Standard curve (8 points) used in each batch of MRM analysis The cost covers a section of 120min LC/MS run, a section of 1-hour labor and a section of reagent kit 2. | $200.00 |
| S16-Nucleoside Modification Analysis | Adenosine and M6A analysis. Analysis of Guanine, Uridine and other bases or nucleosides are also available. The cost covers a section of 10min LC/MS run, a section of 10min labor and a section of reagent kit 1. | $25.00 |
| S17-Peptide fractionation | Peptide fractionation by high pH reversed-phase HPLC. Default use 2 hour gradient for 60 fractions. The cost covers a section of 2-hr HPLC run, a section of 1-hour labor, 4 section of reagent kit 2 and 3 sections of speed vac. | $200.00 |
| S18-PFAS analysis | PFOA/PFOS analysis by MRM (EPA 537.1 or 8327) The cost covers a section of 20-min LC/MS run, a section of 10-min labor and a sectoin of reagnet kit 1. | $40.00 |
| S19-Protein ID without fractionation | Protein identification service The cost covers a section of protein digestion, a section of 1-hr nano-LC/MS run, a section of desalting, a section of Mascot search and a section of scaffold analysis. | $175.00 |
| S20-Protein/compound Molecular Weight Determination | Protein or compound molecular weight measurement by high resolution mode of Q-Tof or QE-HF coupled to UPLC. The cost covers a section of 40-min LC/MS run, a section of 10min labor and a section of reagent kit 1. | $60.00 |
| S21-S1p Analysis | Quantitative analysis of Sphingosine-1-phosphate (S1p) in biological samples. The cost covers a section of 25-min Qtrap LC/MS run, a section of 5-min labor and a section of reagent kit 1. | $40.00 |
| S22-Short Chain Fatty Acid Quantification | SCFA quantitative analysis in biological samples including fecal, serum/plasma, cell lysate, etc. Routine analysis covers C2-C5. Other SCFAs are also available. The cost covers a section of derivatization, a section of Qtrap 25-min LC/MS MRM run, a section of 5min labor time and a section of reagent kit 1. Additional charge will be applied if extra sample preparation is needed. | $55.00 |
| S23-Trace Metal Analysis by ICP MS | Digested solution for metal analysis Standard suite includes Al V Cr Mn Fe Ni Co Cu Zn As Se Mo Cd Sb Sn Pb The cost covers a section 7min ICP MS run, a section of 5min labor and a section of reagent kit 1. Additional fee will be applied if acid digestion is needed. | $18.00 |
| S24-Untargeted Metabolomics/lipidomics | Untargeted metabolomics/lipidomics analysis by; Q-TOF MS. Run with positive and negative modes with RP column. All the features detected by MS will be aligned, normalized and annotated by Agilent software. The cost covers a section of 50min LC/MS run, a section of 10min labor and a section of reagent kit 2. Additional statistical analysis will be provided for fee if requested. | $90.00 |
| S25-Sex Hormones quantification | Sex hormones and related metabolites quantification such as Estrone (E1), Estradiol (E2), 2-hydroxyestradiol, 2-methoxyestradiol,Progesterone, Allopregnanolone, Testosterone, Dihydrotestosterone etc. The cost covers a section of derivatization, a section of 30min Qtrap LC/MS run, a section of 5min labor and a section of reagent kit1. Additional fee will be applied if sample preparation is needed. | $60.00 |
| S26-Tryptophan Cycle analysis | Metabolites quantification related to tryptophan cycle such as tryptophan, kynurenic acid, kynurenine, serotonin, dopamine, norepinephrine etc. The cost covers a section 25min Qtrap LC/MS run, a section of 5min labor and a section of reagent kit 1. Additional charge will be applied if sample preparation is needed. | $40.00 |
| S27-Customized Analysis | The following service are also available in the core. The price will be determined by the number of analytes, sample types, sample preparation etc. Please contact the core for more details. | $0.00 |
| Targeted assay | Ceramide Ceramide-1-Phosphate (C1P) Eicosanoids (prostaglandins, leukotrienes, etc) Endocannabinoids (eCBs) Free fatty acids Lysophosphatidic acid (LPA) – Molecular species Phosphatidic acid (PA) – Molecular species Cholesterol and Oxysterols Glycolipids – GM1, GM2, GM3, GT1, GD1 Phosphatidylinositols (PI, PIP, PIP2, PIP3) Amino Acids Nucleotide and Deoxynucleotide Catecholamines Acyl-CoA cGAMP Antibiotics Primary aromatic amines (PAA) Flux Protein or peptide quantification | |
| Untargeted assay | Global quantitative proteomics by TMT, iTraq or SILAC. |